| 曾莹莹,喻明兰,王婷婷,刘可智,向波.吸烟与C反应蛋白水平的因果关系：一项双样本孟德尔随机化研究[J].四川精神卫生杂志,2024,37(6):567-571.Zeng Yingying,Yu Minglan,Wang Tingting,Liu Kezhi,Xiang Bo,Causal relationship between smoking and level of C-reactive protein： a two-sample Mendelian randomization study[J].SICHUAN MENTAL HEALTH,2024,37(6):567-571 |
| 吸烟与C反应蛋白水平的因果关系：一项双样本孟德尔随机化研究 |
| Causal relationship between smoking and level of C-reactive protein： a two-sample Mendelian randomization study |
| 投稿时间：2024-04-11 |
| DOI：10.11886/scjsws20240411003 |
| 中文关键词: 吸烟 C反应蛋白 全基因组关联研究 孟德尔随机化 |
| 英文关键词:Smoking C-reactive protein Genome-wide association studies Mendelian randomization |
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| 中文摘要: |
| 背景 吸烟与C反应蛋白（CRP）有关，但既往研究结果可能受到混杂因素的影响，CRP在吸烟相关病理过程中的因果关系有待一步探究。目的 利用全基因组关联研究（GWAS）结果数据，探讨吸烟与CRP水平之间的因果关系，为制定相关公共健康政策和吸烟干预措施提供参考。方法 采用CRP以及开始定期吸烟的年龄、开始吸烟、戒烟、日吸烟量四种吸烟表型的GWAS结果数据，选择相互独立且与吸烟及CRP相关的遗传位点作为工具变量。应用逆方差加权法（IVW）及加权中位数法进行孟德尔随机化（MR）分析，对吸烟及CRP的双向因果关系进行探讨。采用Cochran’s Q检验评估每个单核苷酸多态性（SNP）之间的异质性；采用MR多效性残差总和及异常值检测SNP异常值；采用MR-Egger截距测试检验SNP的水平多效性；采用留一法敏感性分析检验MR研究是否受单个SNP的影响。结果 CRP与开始吸烟之间存在双向因果关系（β=0.170，P=0.010）（开始吸烟是暴露因素）、（β=0.040，P=0.001）（CRP是暴露）。结论 吸烟可能会导致CRP水平改变，而CRP水平改变也可能影响个体开始吸烟的倾向。 |
| 英文摘要: |
| Background Previous investigations have illuminated the correlation between smoking and C-reactive protein （CRP）， but previous research findings may be influenced by other confounding factors. The causal relationship of CRP in smoking-related pathological process requires further exploration.Objective To investigate the causal relationship between smoking behavior and CRP by utilizing the cumulative statistical data from existing genome-wide association studies （GWAS）， so as to provide references for formulating relevant public health policies and smoking intervention measures.Methods This research utilized the GWAS summary statistics for CRP and four smoking phenotypes： age of initiation of regular smoking， smoking initiation， smoking cessation and cigarettes per day-selecting independent genetic loci correlated with smoking and CRP as instrumental variables. The study employed the inverse variance weighted method （IVW） and the weighted median approach for two-sample Mendelian randomization （MR） analysis to explore the bidirectional causal relationship between smoking and CRP. The Cochran's Q test was applied to assess heterogeneity among single nucleotide polymorphisms （SNPs）. MR pleiotropy residual sum and outlier was used to detect SNP outliers. MR-Egger intercept test examined the horizontal pleiotropy of SNPs. Leave-one-out sensitivity analysis assessed the impact of individual SNP on the Mendelian randomization results.Results The MR analysis revealed a bidirectional causal relationship between CRP and smoking initiation （β=0.170， P=0.01） （with smoking initiationas the exposure）， （β=0.040， P=0.001） （with CRP as the exposure）.Conclusion Smoking may lead to alterations in CRP levels， while changes in CRP levels could also influence individual's propensity to initiate smoking. |
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