| HUANG Yan-jun,LUO Yong,ZHANG Zhong-nian,Study on dynamic changes of BDNF mRNA and bFGF mRNA in hippocampus of adult rats with focal cerebral ischemia/reperfusion[J].SICHUAN MENTAL HEALTH,2015,28(1):17-21 |
| Study on dynamic changes of BDNF mRNA and bFGF mRNA in hippocampus of adult rats with focal cerebral ischemia/reperfusion |
DOI：10.11886/j.issn.1007-3256.2015.01.006 |
| English keywords:Focal cerebral ischemia/reperfusion BDNF mRNA bFGF mRNA hippocampus Rat |
| Fund projects:四川省卫生厅科研课题(080211) |
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| Objective To study on dynamic changes of BDNF mRNA and bFGF mRNA in hippocampus of adult rats with focal cere-bral ischemia/reperfusion. Methods The animal model of focal cerebral ischemia/reperfusion was made by filament occlusion of the right middle cerebral artery. 108 male Wistar rats were randomly divided into three groups:normal control group(NC group),sham operation con-trol group(SC group),ischemia/reperfusion group(I/R group),each group contain 1,3,7,14,21 and 28d six time points(for each point,n=6). In situ hybrization method was used to detect the number of BDNF mRNA and bFGF mRNA expression positive cells following various time in hippocampus of adult rats. Results Normally a small amount of BDNF mRNA and bFGF mRNA in hippocampus. After focal cerebral ischemia/reperfusion,the number of BDNF mRNA and bFGF mRNA positive cells increased at each time point,reached small peak value after 3 days(P<0. 01). Immunoprecipitates were dyed deeply,after 7 days decreased rapidly,28d decreased to normal level(P<0. 05). Conclusion Focal cerebral ischemia/reperfusion can up-regulated expression of BDNF mRNA and bFGF mRNA,Advantageous to the recovery of neural function after cerebral ischemia and have neuroprotective effect. |
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