| Zhao Rong,Zhu Youwei,Xie Bin,Identification of potential hub genes involved in alcohol use disorder via bioinformatics analysis[J].SICHUAN MENTAL HEALTH,2023,36(3):228-234 |
| Identification of potential hub genes involved in alcohol use disorder via bioinformatics analysis |
DOI：10.11886/scjsws20230331001 |
| English keywords:Alcohol use disorder GEO Differentially expressed genes Hub genes |
| Fund projects:上海市精神卫生中心“飞翔计划”项目（项目名称：人际交互模式对酒精成瘾者脑功能机制的影响研究，项目编号：2022-FX-01） |
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| Background Alcohol use disorder （AUD） is a type of chronic relapsing brain disorder. Genetic factors play an important role in the pathogenesis of AUD. And screening for molecular markers of AUD is of great significance for further elucidating the pathogenesis of the disease， discovering novel therapeutic targets and preventing relapse.Objective To explore relevant hub genes and potential signal pathways associated with the development of AUD through bioinformatics analysis， and to provide a new direction for the prevention and treatment of AUD.Methods The GSE161986 dataset was acquired from the Gene Expression Omnibus （GEO） database. The limma package in R was utilized to identify differentially expressed genes （DEGs）. Gene set enrichment analysis （GSEA） was carried out using the Database for Annotation， Visualization and Integrated Discovery （DAVID）. A protein–protein interaction （PPI） network of DEGs was assessed using the STRING database and visualized by Cytoscape software. Finally， hub genes were validated in GSE44456 dataset.Results A total of 114 DEGs were identified. GSEA revealed that these genes were mainly involved in the regulation of signal transduction， protein binding， membrane trafficking and MAPK signaling pathway. PPI network and validation study indicated that GAD1， TIMP1 and CD44 were potential hub genes involved in AUD.Conclusion Aberrant expression of GAD1 and TIMP1 as well as MAPK signaling pathway may play a key role in the pathogenesis of AUD， and may serve as potential molecular targets for the diagnosis and treatment of AUD. ［Funded by "Flying Project" of Shanghai Mental Health Center （number， 2022-FX-01）］ |
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